Part:BBa_K137053:Design

Constitutive folB expression construct

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We had originally planned on overexpressing the entire folate operon, as well as the individual gene components of the operon. However, we were unable to clone the operon out of the genome successfully.

Source

The original folB gene was extracted from the Lactoccocus lactis subspe. IL1403 genome (ordered from ATCC).